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Promega
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Applied Bioinformatics
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Image Search Results
Journal: Journal of Fungi
Article Title: MAC Family Transcription Factors Enhance the Tolerance of Mycelia to Heat Stress and Promote the Primordial Formation Rate of Pleurotus ostreatus
doi: 10.3390/jof10010013
Figure Lengend Snippet: Amino acid sequence alignment and gene structure diagram of PoMAC1a and PoMAC1b. ( A ) Amino acid sequence alignment. The Cu-fist motifs of PoMAC1 are enclosed in red rectangles, while the REP-I and II motifs are enclosed in blue rectangles. ( B ) The PoMAC1 protein molecular peptide chain and the distribution of various functions. The gray area represents the Cu-fist motif, while the red and blue areas represent the REP-I and II motifs, respectively. The listed amino acid sequences are Cu-fist, REP-I, and II motifs.
Article Snippet: The nuclear localization signals (NLS) of the
Techniques: Sequencing
Journal: Journal of Fungi
Article Title: MAC Family Transcription Factors Enhance the Tolerance of Mycelia to Heat Stress and Promote the Primordial Formation Rate of Pleurotus ostreatus
doi: 10.3390/jof10010013
Figure Lengend Snippet: Verification of OE and RNAi strains. ( A ) Construction method of OE vectors. ( B ) Construction method of RNAi-silencing vectors. ( C ) PCR assay of hyg in P. ostreatus mutant strains, WT, and plasmid. ( D ) qPCR analysis of the relative expression of PoMAC1 in the tested strains. Different letters indicate significant differences for the comparison of samples ( p < 0.05 according to Duncan’s test).
Article Snippet: The nuclear localization signals (NLS) of the
Techniques: Mutagenesis, Plasmid Preparation, Expressing, Comparison
Journal: Journal of Fungi
Article Title: MAC Family Transcription Factors Enhance the Tolerance of Mycelia to Heat Stress and Promote the Primordial Formation Rate of Pleurotus ostreatus
doi: 10.3390/jof10010013
Figure Lengend Snippet: Detection of PoMAC1 mutant strains’ tolerance to 32 °C heat stress. ( A ) Colony morphology of different strains at 32 °C. ( B ) The growth rate of different strains at 32 °C. Different letters indicate significant differences for the comparison of samples ( p < 0.05 according to Duncan’s test).
Article Snippet: The nuclear localization signals (NLS) of the
Techniques: Mutagenesis, Comparison
Journal: Journal of Fungi
Article Title: MAC Family Transcription Factors Enhance the Tolerance of Mycelia to Heat Stress and Promote the Primordial Formation Rate of Pleurotus ostreatus
doi: 10.3390/jof10010013
Figure Lengend Snippet: PoMAC1 participates in the resistance of mycelia to 40 °C heat stress. ( A ) The expression levels of PoMAC1a and PoMAC1b under 40 °C stress at different times. ( B ) The effect of PoMAC1a mutant strains on the recovery growth rate of mycelia after heat stress. ( C ) The effect of PoMAC1b mutant strains on the recovery growth rate of mycelia after heat stress. The values are the mean ±SE of three independent experiments. Different letters indicate significant differences for the comparison of samples ( p < 0.05 according to Duncan’s test).
Article Snippet: The nuclear localization signals (NLS) of the
Techniques: Expressing, Mutagenesis, Comparison
Journal: Journal of Fungi
Article Title: MAC Family Transcription Factors Enhance the Tolerance of Mycelia to Heat Stress and Promote the Primordial Formation Rate of Pleurotus ostreatus
doi: 10.3390/jof10010013
Figure Lengend Snippet: PoMAC1a promotes primordia formation in P. ostreatus . ( A ) The expression patterns of PoMAC1a during different developmental stages. ( B ). The expression patterns of PoMAC1b during different developmental stages. ( C ) Mushroom production of the WT strain and PoMAC1 a mutant strains. ( D ) Mushroom production of the WT strain and PoMAC1b mutant strains. The values are the mean ± SE of three independent experiments. Different letters indicate significant differences for the comparison of samples ( p < 0.05 according to Duncan’s test).
Article Snippet: The nuclear localization signals (NLS) of the
Techniques: Expressing, Mutagenesis, Comparison